Comparison of bacterial communities in roots of selected trees with and without summer truffle (<i>Tuber aestivum</i>) ectomycorrhiza

Abstract In this study, we examined the effect of presence mycorrhiza and ascomata summer truffle ( Tuber aestivum ) on bacterial composition roots from small trees growing in selected sites Nida Basin. Qualitative DNA sequencing methods such as Sanger next-generation (NGS) were used. The method revealed different species compositions between samples where was recorded control samples. Five genera bacteria could be distinguished: Bacillus , Erwinia Pseudomonas Rahnella Serratia among which most numerous Gammmaproteobacteria class) at 32.9%. results obtained by NGS also showed differences depending study sample. Seven Rhizorhabdus Methylotenera Sphingomonas Nitrosospira Streptomyces Methyloceanibacter Niastella dominated sites. Telmatobacter Roseiarcus Granulicella Paludibaculum Acidipila Acidisphaera Aliidongia With method, it is possible to identify microbiome a whole root, while only root fragment can analysed method. These extend scope knowledge preferences certain groups associated with truffles their influence formation truffles. Our may useful selecting monitoring that promote .